Peptide having an affinity for gp120

ABSTRACT

The peptide in this invention is a peptide having affinity to gp120 represented by 
     
       
         H-A1-A2-A3-A4-A5-R (SEQ ID No. 1)  Formula (1)  
       
     
     (in the formula, 
     H means hydrogen, 
     A1 is aspartic acid, lysine, valine, glutamic acid, glycine, asparagine, or tyrosine residue, 
     A2 is valine, aspartic acid, tryptophan, lysine, phenylalanine, isoleucine, leucine, or tyrosine residue, 
     A3 is lysine, valine, aspartic acid, arginine, alanine, or tryptophan residue, 
     A4 is alanine, tryptophan, or glycine residue, 
     A5 is glycine, alanine, valine, leucine, isoleucine, serine, threonine, methionine, asparagine, glutamine, histidine, lysine, arginine, phenylalanine, tryptophan, proline, or tyrosine residue, 
     R is OH derived from carboxyl group or NH 2  derived from acid amide group).

BACKGROUND OF THE INVENTION

1. Field of the Invention

This invention relates to a peptide which has an affinity for gp120, HIV(human immunodeficiency virus) envelope protein.

2. Description of the Related Art

The therapy for HIV infection is usually chemotherapy, such as thenucleotide derivative AZT (3′-azido-3′-deoxythmidine). This AZT therapyor protease inhibitor, which was later developed, prolongs the life ofHIV patients, but there are some problems, these are derived from thechemotherapy itself.

The problems are shown as follows: The first is chronic poisoning due tolong term administration, the second is the appearance of an HIV virusresistant to the medicine during the therapy, the third is theappearance of malignant tumors in prolongation of the HIV patient'slife, the fourth is that the recovery of the immune system can not bemonitored, the fifth is that there is not a method to monitor treatmenteffect, etc. Since such chemotherapy is not basic therapy for HIVinfections, most people anticipate the development of a vaccine.

Generally, the vaccine is an inactive treatment (in active vaccine) of amicrobe of viruses; a weak activity virus which loses pathogenesis or apseudo virus (live vaccine) which has no fatal effects to humans.However, although HIV itself is natively a weak activity virus, it iswell known to stay long after having once invaded the body. In addition,the host cell of HIV is mainly a lymphocyte, which controls the immunesystem; furthermore, HIV spreads over hemophilic patients throughblood-preparation. From these finding, even if it is assumed that weselected either an in-active or a weak vaccine, the development of anHIV vaccine has many problems with safety.

Accordingly, an HIV vaccine is being developed which utilizes a part ofthe HIV envelope protein and inhibits further infection.

From such an idea, many researchers performed an epitope analysis of gp120 in the HIV enveloped protein, and then, watched the V3 region (3rdhypervariable region) of gp120 as an epitope. But it was a truehypervariable region [Palker T. J., et al., Proc. Natl. Acad. Sci. USA85:2709-2713, 1988; Rusche J. R., et al., ibid 85:3198-3202,1988;Gouddsmit J., et all., ibid 85:4478-4482.1988; Matsushita S., et al., JVirol. 62:2107-2114,1988]. After this, a vaccine which used a part ofthis region as antigen was administrated to an HIV infected monkey as aninfection inhibitory experiment, but the effection has not yet beenreported.

As well as this, Tam et al. devised further antigenecity for theabove-mentioned peptide antigen (Tam et. al., Japanese patentpublication(Tokuhyo) No. H 3-503539), but have not yet had successbecause in most parts of the V region, particularly in the V3 which is aconvenient region for antibody preparation, mutation or deletion occurs.

In addition, a neutralized antibody, which inhibits the infectionagainst lymphocyte, is developed. For example, in Japanese PatentApplication No. 63-171385, after the production of a monoclonal antibodyby using a part of the above mentioned peptide as antigen, a method isreported, which produces anti HIV chimera antibodies on hybridizationdue to genetic engineering at the level of the protein as the Fab′itself. But, although with such neutralizing antibodies it is possibleto inhibit HIV infection to the lymphocyte at laboratory level, anantibody that can be used practically has not yet been developed.

As mentioned above, chemotherapy has some problems; drug tolerance inthe virus and side effects in the host, another idea to solve theproblem of removing the virus from the body is by plasmapheresis.Although this method to remove the HIV virus by using a pore sizemembrane filter (smaller than virus size) for plasmapheresis has beendefinitely proposed it is not yet possible to make a uniform pore sizemembrane. It is also possible that the pores will become clogged duringplasmapheresis resulting in the deterioration of the membrane due topressure. As mentioned above there are many technical problems whichhave to be settled. So, a method to use CD4 derived from humanlymphocyte having specific affinity to HIV, as absorbed carrier incolumn for plasmapheresis is also proposed. It cannot be used as amedical procedure because of the lost affinity due to decay by autoclavetreatment. In addition, there are also methods using thermostablemolecules, a high molecule polymer or color ligand as an affinitycarrier to HIV. However, as these molecules do not originally havespecific binding ability to HIV, they cannot be used because they bindto blood ingredients faster than to HIV.

In this way, aiming at the development of an HIV treatment medicine,research to produce a vaccine and neutralizing antibody is flourishing,but useful medicine has not yet been developed.

The inventors paid attention to this present situation and developed asuperior peptide to have the same degree or more affinity for gp120compared to antibodies and to be resistant to autoclave treatment, andhave already made a patent application (Japanese Patent Application No.H 8-351474 and Japanese Patent Application No. 8-351475). This peptidebasically consists of a three amino acid sequence, but from a study ofthe sequel, we found that an affinity to gp120 of this peptidedeteriorated by number and a kind of the amino acid which ranged in it.So, we knew that we needed to develop a more stable peptide.

SUMMARY OF THE INVENTION

In view of the above, an object of this invention is to provide a novelpeptide which has an affinity for gp120, HIV envelope protein, withexcellent stability, and a variety of usabilities using the peptide.

DESCRIPTION OF THE PREFERRED EMBODIMENTS

FIG. 1 is a graph that shows result of EXAMPLE 8.

FIG. 2 is a photograph by electron microscope that shows the result ofEXAMPLE 15.

BEST MODE FOR CARRYING OUT THE INVENTION

The No. 1 peptide in this invention that could solve the above subject;

A peptide having an affinity to gp120 represented by formula (1):

H-A1-A2-A3-A4-A5-R (SEQ ID No. 1),

(in the formula,

H means hydrogen,

A1 is aspartic acid, lysine, valine, glutamic acid, glycine, asparagine,or tyrosine residue,

A2 is valine, aspartic acid, tryptophan, lysine, phenylalanine,isoleucine, leucine, or tyrosine residue,

A3 is lysine, valine, aspartic acid, arginine, alanine, or tryptophanresidue,

A4 is alanine, tryptophan, or glycine residue

A5 is glycine, alanine, valine, leucine, isoleucine, serine, threonine,methionine, asparagine, glutamine, histidine, lysine, arginine,phenylalanine, tryptophan, proline, or tyrosine residue,

R is OH derived from carboxyl group or NH₂ derived from acid amidegroup).

Accordingly, the No. 1 peptide in this invention is a 5 amino acidsequence that was constituted by A1, A2, A3, A4 and A5 as describedabove, and all of the peptide including such amino acid sequencescontained by the range of this invention. Thus, a peptide having anaffinity to gp120 represented by

A1′-A2-A3-A4-A5-R-(SEQ ID No 2),  Formula (2)

(in the formula,

A1′ means aspartic acid, lysine, valine, glutamic acid, glycine,asparagine, or tyrosine residue, or polypeptide residue that anarbitrary amino acid stood in line in the N-terminal side from thisamino acid, A2, A3, A4, A5 and R have the same meaning as above)

or

H-A1-A2-A3-A4-A5′-R (SEQ ID No. 3),  Formula (3)

(in the formula,

A5′ means glycine, alanine, valine, leucine, isoleucine, serine,threonine, methionine, asparagine, glutamine, histidine, lysine,arginine, phenylalanine, tryptophan, proline, or tyrosine residue, orpolypeptide residue that an arbitrary amino acid stood in line in theC-terminal side of this amino acid, H, A1, A2, A3, A4 and R have thesame meaning as the above)

is entirely one aspect of the present invention.

Then, the No. 2 peptide that could solve the above subject is;

a peptide having an affinity to gp120 represented by

H-a1-a2-a3-a4-a5-R (SEQ ID No. 4),  Formula (4)

(In the formula,

H means hydrogen,

a1 is tyrosine, arginine, phenylalanine, glycine, tryptophan, histidine,or aspartic acid residue,

a2 is arginine, tyrosine, tryptophan, alanine, valine, glutamine,histidine, or lysine residue,

a3 is lysine, tyrosine, arginine, glutamic acid, methionine, ortryptophan residue,

a4 is glycine, alanine, valine, leucine, isoleucine, serine, threonine,methionine, asparagine, glutamine, histidine, lysine, arginine,phenylalanine, or tryptophan residue

a5 is glycine, alanine, valine, leucine, isoleucine, serine, threonine,methionine, asparagine, glutamine, histidine, lysine, arginine,phenylalanine, tyrosine, or tryptophan residue,

R is OH derived from carboxyl group or NH₂ derived from acid amidegroup).

Accordingly, the No. 2 peptide in this invention is a 5 amino acidsequence that was constituted by a1, a2, a3, a4 and a5 as describedabove, and all of the peptide including such amino acid sequencescontained by the range of this invention. Thus, a peptide having anaffinity to gp120 represented by

a1′-a2-a3-a4-a5-R (SEQ ID No. 5),  Formula (5)

(In the formula,

a1′ means tyrosine, arginine, phenylalanine, glycine, tryptophan,histidine, or aspartic acid residue, or polypeptide residue that anarbitrary amino acid stood in the N-terminal side from this amino acid,a2, a3, a4, a5 and R have the same meaning as above.)

or

H-a1-a2-a3-a4-a5′ (SEQ ID No. 6),  Formula (6)

(In the formula,

a5′ is glycine, alanine, valine, leucine, isoleucine, serine, threonine,methionine, asparagine, glutamine, histidine, lysine, arginine,phenylalanine, tyrosine, or tryptophan residue, or polypeptide residuethat an arbitrary amino acid stood in line in the C-terminal side ofthis amino acid, H, a1, a2, a3, and a4 have the same meaning as above)

is entirely one aspect of the present invention.

In addition, this invention includes a compound which is a macromoleculecompound that has a functional group and/or medicine bound to the No.1or No.2 peptide as mentioned above, or a pharmaceutically acceptablesalt thereof.

These peptide compounds or materials including them have an affinity togp120.

Furthermore, the above peptides or a pharmaceutically acceptable saltthereof, and the composition including pharmaceutically acceptablecarrier and/or medicinal bioactivity, are contained in this invention.Also, various aspects, such as; the detection, the diagnosis and theremoval to viruses such as HIV by using the above peptide (for example,using the HIV diagnosis or the detection kit contained it, HIV absorbingand removal carrier, and therapy by plasmapheresis) are contained inthis invention.

However, the above mentioned “peptide” that is used in this inventioncontained in the C-terminal peptide is COOH, acid amide and ester etc.,and particularly, so long as we do not specify, it contains an aminoacid number (oligopeptide) of less than 10, or a polypeptide of morethan this.

An amino acid in the above mentioned peptide contains the derivativesthat are protected by the protecting functional group. As such aminoacid derivatives, it is marked by substitution or modification withoutexchanging the peptide structure; exchanging the length of the carbonchain etc., or the protecting amino acid derivatives corresponding tovarious amino acids, but all of these various amino acids can be used inthis invention. For example, as tyrosine derivatives, there is2,6-dichloro-L-tyrosine having chloride in the side chain,p-Nitro-L-phenylalanine that hydroxyl group of p-side in phenylalaninewas substituted Nitroyl group, and 4-chloro-L-phenylalanine that thehydroxyl group was substituted chloride, etc. In addition, as valinederivatives, there are Norvaline: N-α-L-norvaline, or MeVal:N-α-L-valine, etc.

The reason that conventional medicine, such as a vaccine or neutralizingantibody can not be used clinically is that the HIV region the body canrecognize as antigen is the V (hypervariable region) region in theenvelop gp120, and this is the most problematic. So, the inventorsresearched a peptide which had a specific infinity to gp120, and as aresult, developed a superior peptide and have already applied for apatent (Japanese Patent Application No. H 8-351474 and Japanese PatentApplication No. H 8-351475). They developed a peptide which has a highspecific affinity to gp120, of the same affinity or more compared toantibody, and which is additionally resistant to heat with a highpressure, such as in autoclave treatment.

However, from research after this, we found that the above-mentionedaffinity to gp120 deteriorated by number and there was a kind of aminoacid which ranged in it. So, we continued to research further to supplya more stable peptide, and make this invention complete.

In addition to the above, “affinity”, this invention shows a specificand tight bond with weak interaction, such as electrostatic interaction,hydrogen bond, Van der Waals attraction, hydrophobic bond and etc,gathered except a covalent bond.

The peptide in this invention is constituted as mentioned above and isfundamentally shown as 5 amino acid residues which appear in;

H-A1-A2-A3-A4-A5-R (SEQ ID No. 1),  {circle around (1)}formula (1)

(in formula, A1, A2, A3, A4, A5 and R, the meanings are the same asbefore) or,

H-a1-a2-a3-a4-a5-R (SEQ ID No. 4),  {circle around (2)}formula (4)

(in formula, a1, a2, a3, a4, a5 and R, the meanings are the same asbefore).

This peptide has a molecule separate in each and is not (or in peptide),the amino acid sequence mentioned above {circle around (1)}peptide,

A1′-A2-A3-A4-A5 (SEQ ID No. 2);  formula (2)

or

A1-A2-A3-A4-A5′ (SEQ ID No. 3)  formula (3)

or above-mentioned {circle around (2)}peptide includes the sequencewhich lined up from N-terminus.

a1′-a2-a3-a4-a5 (SEQ ID No. 5);  Formula (4)

or

a1-a2-a3-a4-a5′ (SEQ ID No. 6)  Formula (5)

(in formula, A1′, A2, A3, A4, A5′, a1′a2, a3, a4, a5′, the meanings arethe same as before).

Of course, in A1′-A2-A3-A4-A5′ (SEQ ID Nos. 1-3) or a1′-a2-a3-a4-a5′(SEQ ID Nos. 4-6), there includes peptides which lined up repeatedly bythis order. In brief, this invention includes all of the peptides whichconsist of 5 amino acid residues and have an affinity to gp120.

The peptide in this invention can be synthesized by conventionalmethods; For example, the first of this invention is constituted fromA1-A2-A3-A4-A5 (SEQ ID No. 1), is synthesized and the A5 glycineresidue, carboxyl of N-protective glycine is bound to some carrier, suchas insoluble resin, which has a functional group that can couple tocarboxyl. After this, the protected amino acid in each, from A2 to A5,is bound in order by a solid phase synthetic method, and the peptideshown in this invention can be obtained by reacting the above mentionedinsoluble resin and eliminating the protection of the amino acid.

In addition to the above, an end of carboxyl in A5 amino acid residue isfree(R, that is to say is equivalent to —OH), or is substituted withacidic amide(R, that is to say is equivalent to —NH₂). Then, anend-carboxyl of A5 with carboxyl of spacer together, bound thiscarboxyl, binds a synthetic macromolecule, bio-macromolecule, andutilized well macromolecular compound, which has a functional group (asin the postscript). In addition to the above, amino acid used by theabove mentioned solid phase synthetic methods is common to L type or Dtype, but L type is more pleasing.

In the case of the above, the carrier used solid phase synthetic methodhas carboxyl group of N-protected glycine of C-terminus through theamino group, or if it can bind to this carboxyl group and can eliminateafter the binding, it is not limited at all. For example,chloromethyl-resin (chloromethylpolystyrenedivinylbenzene),oxymethyl-resin (oxymethylpolystyrenedivinylbenzene) and others areexemplified. Then, resin of 4-(oxymethyl)phenylacetamidemethyl-resin,4-(2′,4′-Dimethoxyphenyl-aminomethyl)phenoxyacetamidemethyl-resin andetc., benzyloxybenzylalcohol-resin, benzhydrylamine-resin,insoluble-resin which has amino group, methylbenzhydrylamine-resin,aminomethylphenoxymethyl-resin, dimethoxybenzhydrylamine (DMBHA)-resin,and the derivatives are exemplified. In these, benzhydrylamine-resin,methylbenzhydrylamine-resin, aminomethylphenoxymethyl-resin andDMBHA-resin can be get directly amide by cleavage after the binding.Judging from the yield, use of aminomethyl-resin is desirable.

In addition, a spacer which has a functional group binding with carboxylgroup and has a carboxyl group are picked up which can transformp-carboxymethylbenzylester-resin to carboxyl group of glycine as in theexample.

Moreover, “protecting amino acid” in the case of the above meansprotected amino acid with protecting group by conventional method. Tosynthesize the peptide invented, it is pleasing to use either of theprotecting groups shown in the following examples.

In this example, the protecting group of α-amino in amino acid is Boc(t-butoxycarbonyl) or Fmoc (9-fluorenylmethoxycarbonyl); protectinggroup of ξ-amino in lysine is Z (benzyloxycarbonyl),Cl.Z(2-chlorobenzyloxycarbonyl), Boc, Npys(3-nitro-2-pyridinesulfonyl);protecting group of hydroxyl group in tyrosine is Bzl(benzyl),Cl₂.Bzl(2,6-dichlorobenzyle), or t-Bu(tert.-butyl) are exemplified, butit uses the peptide synthesis well even if the hydroxyl group of thistyrosine is not protected by the above mentioned protecting group;protecting group of guanidino group in arginine is Tos(tosyl),NO₂(Nitro), Mtr(4-methoxy-2,3,6-trimethylbenzenesulfonyl), orpmc(2,2,5,7,8-pentometylchloroman-6-sulfonyl); protecting group ofcarboxyl group in glutamic acid is Bzl ester, t-Bu ester, cHx(cyclohexlylester); protecting group of amide group in glutamine isTrt(trityl) is picked up, but it can be used even if glutamine is notprotected by this protecting group; the protecting group of indole groupin tryptophan is formyl group or Boc, but it can be used even iftryptophan is not protected by this protecting group. These protectinggroups can be used to select the most suitable protecting amino acidaccording to the condition of the peptide synthesis.

A binding of protecting amino acid can be carried out with the usualcondensed-polymerized method, as for example, DCC(N′,N-dicyclohexylcarbodiimide)method(R. B. Merrified: Biochemistry, 3,1385, 1964), DICDI(N′,N-diisopropylcarbodiimide) method(D. Sarantakis,et al: Biochem. Biophys. Res. Commun., 73,336,1976), active-estermethod(F. Weygan, et al.: Z. Naturforsch., B, 21, 1141,1966), mixturedor symmetrical acetic anhydride method(D. Yamashiro, et al.: Proc. Natl.Acad. Sci. USA, 71, 4945,1945) carbonyldiimidazole method,DCC-HOBt(1-hydroxybenzotriazole) method(Keonig, W., et al.; Chem. Ber.,103: 788,1970), diphenylphosphorylazide method, etc. But particularly,DCC, DCC-HOBt, DICDI-HOBt and symmetric acidic anhydride method arerecommended. These condensed—polymerized reactions are usually performedin organic solvent of dichloromethane, dimethylformamide etc. or intheir mixture solution.

As in the case above, reagents to eliminate the protecting group ofα-amino group, trifluoroaceticacid(TFA)/dichloromethane, HCl/dioxane,piperidine/dimethylformamide(DMF), etc. are used and can be selectfreely according to the kind of protecting group. Then, the degree ofprogress in the condensed reaction on each step of synthesis can beconfirmed by the ninhydrin reaction method (E. Kaiser, et al., Anal.Biochem., 34:595,1970).

In this way, we can get protected peptide resin which has an amino acidsequence represented with an upper expression, and after this, can get asuitable peptide by elimination of the protecting group from theinsoluble resin and amino acid. In the case using chloromethyl resin asinsoluble resin, it is treated by hydrogen fluoride with anisole. Then,in this case used benzyloxybenzylalcohol-resine, benzhydrylamine-resin,and DMBHA resin(Funakoshi, S., J. Chem. Soc., Chem. Commun., 198:382,1988), this resin and protecting group can be eliminated at sametime by treatment of hydrofluore, TFMSA (Trifluoromethanesulfonicacid),TMSOTF (Trimethylsilyltriflate), TMSBr (Trimethylsilylbromide) andothers.

The peptide that is obtained in this way can be purified by variousmethods; chromatography (gel, ion-exchange, hydrophobicity, adsorption,reverse phase), electrophoresis and ultrafiltration.

Also included in this invention is a peptide that was substituted on asimilar protein(active center or binding domain of antibody, receptor,enzyme and etc.) by a gene recombination method in the case of abovepeptide. For example, if we produce human anti-gp120 antibody by generecombinant method, we produce the above-mentioned peptide which isbased on the U.S. Pat. No. 114,632. Namely, this peptide is transducedamino acids of hypervariable cluster in CDR (complementary determinationregion, VH31 to VH35)-1 and CDR-2(VH50 to 52, and/or VH58 to 60), whichrelates recognition of epitope during the V region in the humanimmunoglobin gene (Ohno, S., Marl, N. & Matunaga, T.; Proc. Natl. Acad.Sci. USA. 82, 2945, 1985).

In this way, the peptide of this invention can produce specific bindingto, gp120 accordingly to its purpose, substituting the gene recombinantmethod.

For example, the first peptide in this invention is shown from table 1to 2 and at same the second peptide is shown from table 3 to 4,respectively. As in the case above, the table shows an effectiveagglutinin test and neutralizing activity. Here, No. 24 in Table 1 isequivalent to the first peptide, and also matches the second peptide.

TABLE 1 Agglu- Neutra- tinin lizing No. A1 A2 A3 A4 A5 test activity 1Asp Val Lys Ala Gly * 2 Asp Lys Val Ala Gly * 3 Lys Val Asp Ala Gly * 4Val Lys Lys Ala Gly * 5 Asp Asp Lys Ala Gly * 6 Lys Asp Asp Ala Gly * 7Val Asp Asp Ala Gly * 8 Asp Val Asp Ala Gly * 9 Val Val Lys Ala Gly * *10 Val Val Asp Ala Gly * 11 Lys Val Val Ala Gly * 12 Asp Asp Val AlaGly * * 13 Asp Asp Asp Ala Gly * 14 Val Lys Val Ala Gly * 15 Asp Try LysAla Ala * 16 Asp Phe Lys Ala Gly * 17 Asp Trp Lys Ala Gly * 18 Asp ValArg Ala Gly * 19 Glu Val Lys Ala Gly * 20 Gly Gly Asp Val Lys Ala Gly *21 Gly Asp Val Lys Ala Gly * 22 Val Ile Asp Ala Gly * 23 Val Leu Asp AlaGly * 24 Gly Val Lys Ala Gly * 25 Asp Val Lys Trp Ala * 26 Asp Val LysGly Lys * 27 Asp Val Lys Gly Trp * Nos. 1-19 and 22-27 of Table 1correspond to SEQ ID No. 1. No. 20 of Table 1 corresponds to SEQ ID No.7. No. 21 of Table 1 corresponds to SEQ ID No. 8.

TABLE 2 agglutinin neutralizing No. A1 A2 A3 A4 A5 test activity 28 AspVal Ala Ala Gly * 29 Asp Val Lys Gly Leu * 30 Asp Val Lys Gly Pro * 31Asp Val Lys Ala Val * 32 Asp Val Lys Ala Ile * 33 Asp Val Lys Ala Ser *34 Asp Val Lys Ala Thr * 35 Asp Val Lys Ala Met * 36 Asp Val Lys AlaGln * 37 Asp Val Lys Ala Asn * 38 Asp Val Lys Ala His * 39 Asp Val LysAla Arg * 40 Asp Val Lys Ala Phe * Nos. 28-40 of Table 2 correspond toSEQ ID No. 1.

TABLE 3 Agglutinin Neutralizing No. a1 a2 a3 a4 a5 test activity 1 PheTyr Arg Lys Ala * * 2 Tyr Arg Arg Ala Ala * 3 Trp Trp Glu Ala Ala * * 4Tyr Gln Glu Ala Ala * 5 Gly Tyr Tyr Lys Ala * * 6 Trp Trp Lys AlaAla * * 7 Tyr Tyr Arg Ala Ala * 8 Phe Arg Lys Ala Ala * 9 Tyr Tyr LysLys Ala * * 10 Tyr Tyr Lys Leu Leu * 11 Tyr Arg Lys Ala Ala * * 12 TyrTyr Lys Ala Ala * * 13 Arg Tyr Lys Ala Ala * * 14 Phe Tyr Arg Ala Ala *15 Tyr Ala Lys Ala Ala * * 16 Tyr Tyr Glu Ala Ala * 17 Tyr Trp Lys AlaAla * 18 Gly Tyr Tyr Lys Ala Ala * 19 Typ Tyr Lys Ala Ala * 20 Tyr GlnLys Ala Ala * 21 His Tyr Lys Ala Ala * 22 Tyr Arg Tyr Ala Ala * * 23 TyrTyr Met Ala Ala * 24 Tyr Val Lys Ala Ala * 25 Gly Tyr Ala Tyr Arg Lys *26 Arg Arg Trp Ala Tyr * * 27 Arg Tyr Tyr Lys Ala Ala * Nos. 1-17, 19-24and 26 of Table 3 correspond to SEQ ID No. 1. No. 18 of Table 3corresponds to SEQ ID No. 9. No. 25 of Table 3 corresponds to SEQ ID No.10. No. 27 of Table 3 corresponds to SEQ ID No. 11.

TABLE 4 Agglutinin Neutralizing No. a1 a2 a3 a4 a5 test activity 28 TyrLys Lys Ala Ala * 29 Tyr His Lys Ala Ala * * 30 Asp Tyr Lys Ala Ala * 31Tyr Tyr Lys Trp Ala * 32 Tyr Tyr Lys Gly Ala * 33 Tyr Tyr Lys Ala Gly *34 Tyr Tyr Lys Lys Ala * 35 Tyr Tyr Lys Val Ala * 36 Tyr Tyr Lys IleAla * 37 Tyr Tyr Lys Ser Ala * 38 Tyr Tyr Lys Thr Ala * 39 Tyr Tyr LysMet Ala * 40 Tyr Tyr Lys Gln Ala * 41 Tyr Tyr Lys Asn Ala * 42 Tyr TyrLys His Ala * 43 Tyr Tyr Lys Phe Ala * 44 Tyr Tyr Lys Trp Ala * 45 TyrTyr Lys Arg Ala * 46 Tyr Tyr Lys Ala Val * 47 Tyr Tyr Lys Ala Ile * 48Tyr Tyr Lys Ala Ser * 49 Tyr Tyr Lys Ala Thr * 50 Tyr Tyr Lys Ala Met *51 Tyr Tyr Lys Ala Gln * 52 Tyr Tyr Lys Ala Asn * 53 Tyr Tyr Lys AlaHis * 54 Tyr Tyr Lys Ala Phe * 55 Tyr Tyr Lys Ala Trp * 56 Tyr Tyr LysAla Arg * Nos. 28-56 of Table 4 correspond to SEQ ID No. 4.

A sign of each amino acid formula shows the amino acid residue by theinternationally approved characters, the details are as follows:

Tyr: Tyrosine

Lys: Lysine

Trp: Tryptophan

Arg: Arginine

Glu: Glutamic acid

Gin: Glutamine

His: Histidine

Ala: Alanine

Phe: Phenylalianine

Gly: Glycine

Met: Methionine

Asp: Aspartic acid

Asn: Asparagine

Val: Valine

Ser: Serine

Cys: Cysteine

Thr: Threonine

Ile: Isoleucine

Lcu: Leucine

Pro: Proline

A peptide having such an amino acid sequence shows a superior affinityto gp120, and can be utilized effectively as an anti-HIV medicine bytaking a form of chemical compound or composition shown as follows.

A compound of this invention is matter that binds a high molecularchemical compound and/or medicinal activator functional group, and thisinvention includes the salts to be admitted as medicine.

For example, as pharmaceutically acceptable salts here, followingintoxicant salts in common use is put up.

{circle around (1)}As salts with bases such as inorganic bases, thereare alkali metal salt (for example, sodium salt and potassium salt),alkaline earth metal salt (for example, calcium salt, magnesium salt)and ammonium salt; {circle around (2)} as salts with such as organicbases, there is salts of organic amines (for example, triethylamine,pyridine, picoline, ethanolamine, triethanolamine, dicyclohexylamine,N,N′-dibenzylethylenediamines), etc; {circle around (3)} as salts withacids such as inorganic acids, there are hydrocholic acid, hydrobromicacid, phosphoric acid, nitric acid, sulfuric acid; {circle around (4)}as salts with acids such as organic acids, there are organic carboxylicacid (for example, acetic acid, propionic acid, maleic acid, succinicacid, malic acid, citric acid, tartaric acid and salicylic acid),organic sulfonic acid (for example, methanesulfonic acid,p-toluenesulfonic acid), glyconic acid (glucuronic acid, galactonicacid, gluconic acid, ascorbic acid, and others).

Then, “macromolecule compound having functional group” used on thisinvention is not particularly limited if it can bind the peptide of thisinvention, for example, the following are listed.

(1) Synthetic Polymer

It is selected voluntarily among the inside of linear, branched andcyclic, as polymer in case of above. For example, it can be used as aninsoluble solid phase carrier of amino acidic homopolymer of polylysineand polyglutamic acids, or cyclic polyamine, cyclodextrin, cyclicpeptide and then polystylene, polypropyrene, nylon, silica-gel,polyethyleneglycol, cellulose, polyacrylamide and others.

A branched polymer in these is higher than the usual homopolymer oncontent of functional groups per unit because of divergence at one partin each. For example, it seems to be the lysine core indicated byDenkewalter and is a polymer that is based on the same molecular chain,more than two, derived from a core molecule having at least two or morehaving functional groups U.S. Pat. No. 4,289,872; or it is a starburstdendrimer that the polymer size is regulated closely because the samemolecule reacts continuously, proposed by Tomalia and et al; or it isthe molecule that size was formed irregular, by which the same ordifferent molecule reacts to discontinuity. In addition, ahomo-/branched-polymer as in the case of above does not always to need acarrier which has enough size, and it has a monomer of around 3 thatdoes not usually seem to be recognized as a core, and it is not limitedby the size or introduction number. However, in the case when it isintroduced to numerous peptide formulas, use of divergence numericalpolymer is recommended but even if it is anything polymer. When thepeptide of this invention is bound (to the above mentioned polymer) itis possible that it is synthesized and just grown directly/indirectlyfrom the branched functional group, or that it is conjugateddirectly/indirectly from the functional group of the polymer to a newseparate synthetic peptide.

Moreover, for binding cyclic polymer of cyclic polyamine, cyclodextrin,and cyclic peptides, it is possible to synthesize directly and make thepeptide of upper expression from the same functional group, or to binddirectly/indirectly to a new synthetic peptide separately or to afunctional group of the cyclic polymer. Then, to bind the insolublecarrier of silica gel etc., after it is introduced to the samefunctional group carrier in advance, it can be synthesized and just growdirectly peptide of upper expression from the functional group, orconjugate directly/indirectly from the functional group of insolublecarrier to the new synthetic peptide separately. In addition, theparticular size and shape of the carrier having this same functionalgroup is not limited, and selection and utilization of: spherical,hollow fibrous, fibrous shapes can be made according to their purposeand then, it is not limited at all by size and shape and can beintroduced to several functional groups.

(2) Biopolymer

As above mentioned biopolymer, there are, for example, linear polymerlike polysaccharide, such as heparin, hyaluronic acid, chitosan, chitin,etc.; proteins of proteoglycans, peptide hormone; gelatin, albumin,antibody, and antibody's fragments, etc.

The size of linear polymer in these can be appropriately selected,according to the purpose of use, and includes some monomer of around 3that does not usually seems to be recognized as a polymer, but is notlimited at all by the size or number of functional groups. For binding apeptide or upper expression to this linear polymer, you may directlysynthesize and just grow it from the same functional group, or maydirectly/indirectly conjugate a new synthetic peptide separately to thefunctional group of the linear polymer.

In addition, when peptide hormone and protein is bound to the polymer,it is possible that either end of the peptide of upper expression isbound to cysteine and do disulfide binding with the residue of cysteinein the above mentioned peptide hormone/protein, or is conjugateddirectly/indirectly to the functional group in the above mentionedpeptide hormone/protein with functional group peptide in upperexpression. In this way, we can freely select these binding methodsaccording to the purpose of use, and also it is the same even if thekind and number of peptide shown by upper expression is transduced.

Moreover, an active medicine used by this invention is, for example, AZTof nucleoside derivative known as HIV inhibitor,3,4-Duhydroxy-2,5-di[N-methyl-(2-pyridylmethyl)carbamoyl]valylamino]-1,6-diphenylhexanes known as protease inhibitoragainst HIV. These active medicines can be used to produce specificmedicines against HIV. Accordingly, such a medicine is useful as thetreatment medicine that can specifically treat HIV.

Furthermore, the range of this invention also includes a compositionthat contains a salt and a carrier pharmaceutically permitted asmedicine and/or active medicine.

As mentioned above [the pharmaceutically permitted carrier] can selectthe appropriate use of an excipient (disintegrator, lubricant, expanderetc.), color additives, preservative, stabilizer and other carriers incommon use. The following have definitely been shown crystal cellulose,calcium carmelose, sodium carmelose, hydroxypropylcellulose,hydroxypropylmethylcellulose, ethylcellulose, magnesium stearate, talc,light anhydrouse silicic acid, food color agents, essential oil, etc.

Based on the following enforcement examples, we will describe thedetails of this invention. However, the following enforcement exampledoes not limit this invention, the technological range of this inventionincludes all enforcing changes within the range that does not deviatefrom the purpose of the postscript.

Synthesis 1: Compound which was Bound Polyethylene Glycol with thePeptide in this Invention

After induction of carboxyl group by which was reacted anhydridesuccinic acid against a hydroxyl group of polyethylene glycol (MW.20,000), it is reacted MBS (m-maleimide benzoyl-N-hydroxysuccinimide),and we synthesized maleimide polyethylene glycol.

Then, we synthesized peptide-polyethylene glycol binding compound, bywhich performed peptide binding to peptide induced cysteine againstC-terminal of No.1 peptide shown by Table 1 mentioned above. Aftersuspending this compound in phosphate buffer solution, it was purifiedby gel chromatography and affinity chromatography by gp120 conjugatedcarrier, and then, polyethylene glycol binding compound to the peptidewas synthesized.

Synthesis 2: Compound which was Bound Cyclodextrin with the Peptide inthis Invention

After induced carboxyl group by which was reacted anhydride succinicacid against a hydroxyl group of α-cyclodextrin, it was reactedMBS(m-maleimide benzoyl-N-hydroxysuccineimide), and we synthesizedmaleimide cyclodextrin.

On the other hand, after synthesized a peptide which was performedpeptide binding to C-terminus of cysteine of No.12 peptide on Table 3mentioned above, it reacted to the above maleimide cyclodextrin, and wesynthesized a cyclic compound that cyclodextrin binding compound to thepeptide.

Synthesis 3: Compound (1) which was Bound Branched Polymer with thePeptide in this Invention

Branched polymer binding compound was synthesized by extending No.12peptide the above Table 3 from N-end amino acid MAPs (Multiple antigenicpeptide). After the compound was suspended in phosphate buffer, it waspurified through gel chromatography and affinity chromatography by gp120conjugated carrier, and we synthesized branched polymer binding compoundto the peptide in this invention (2).

Synthesis 4: Compound (2) which was Bound Branched Polymer with thePeptide in this Invention

Branched polymer binding compound was synthesized by extending No.12peptide on the above Table 3 from N-end amino acid MAPs (Multipleantigenic peptide). After the compound was suspended in phosphatebuffer, it was purified by through gel chromatography and affinitychromatography by gp120 conjugated carrier, and we synthesized branchedpolymer binding compound to the peptide in this invention (2).

Synthesis 5: Compound which was Bound AZT with the Peptide in thisInvention

After preparing mixed anhydride to react isobutyl chloroformate withbromoacetic acid, we synthesized bromoacetylester-AZT by esterification,of the hydroxyl group of AZT. On the other hand, after synthesizing bysolid phase method binding the peptide that cysteine was bound C-endamino acid of No. 12 peptide on the above Table 3, it was reacted withthe above bromoacethylestel-AZT, and then we obtained AZT bindingcompound, which is a cross-linking with this peptide.

Synthesis 6: Compound which was Bound Inactive Alkaline Phosphatase withthe Peptide in this Invention

We synthesized maleimide alkaline phosphatase, by which inactivealkaline phosphatase (Alp) was reacted MBS (maleimidebenzoile-N-hydroxysuccinimide). Then, No.8 peptide on the above Table 3induced cysteine was bound with the maleimide group, and we synthesizedinactive alkaline phosphatase binding compound. An inactive form ofalkaline phosphatase was identified by the confirmation that there wasno production of p-nitrophenol when using p-nitrylphenyl phosphate as asubstrate.

Synthesis 7: An Absorbing and Removing Carrier (1) which was BoundSephadex 6 MB with the Peptide in this Invention

No.1 peptide on the above Table 1 was bonded covalently to Sephadex 6 MBby intermediating an activated spacer beforehand, and peptide/Sepharose6 MB(1 μmol of gp120/ml in bed volume) was prepared. Unreactive peptidewas centrifuged at room temperature for 10 minutes(12,000 rpm) by usingphosphate buffer and was removed by repeating the absorbing and removingthe preparation to the supernatant.

Synthesis 8: An Absorbing and Removing Carrier (2) which was BoundSephadex 6 MB with the Peptide in this Invention

No.12 peptide on the above Table 3 was done covalent bond to Sephadex 6MB by intermediating an activated spacer beforehand, andpeptide/Sepharose 6 MB(1 μmol of gp120/ml in bed volume) was prepared.Unreactive peptide was centrifuged at room temperature for 10minutes(12,000 rpm) by using phosphate buffer and was removed byrepeating the absorbing and removing the preparation to the supernatant.

Synthesis 9: an Absorbing and Removing Carrier which was Bound Sephadex4B with the Peptide in this Invention

No.12 peptide on the above Table 3 was done covalent bond to Sephadex 4Bby intermediating an activated spacer beforehand, and thepeptide/Sepharose 4B(1 μmol of gp120/ml in bed volume) was prepared.Unreactive peptide was centrifuged at room temperature for 10minutes(12,000 rpm) by using phosphate buffer and was removed byrepeating the absorbing and removing the preparation to the supernatant.

Synthesis 10: an Absorbing and Removing Carrier which was BoundCellulose Type Carrier with the Peptide in this Invention

No.12 peptide on the above Table 3 was done covalent bond to aperoxycellulose carrier, and it was prepared by washing carefully withsodium hydrogen carbonate buffer(pH8) and phosphate buffer(pH4) afterblocking by glycine. We prepared an absorbing and removing carrier whichwas bound cellulose series carrier with the peptide.

EXAMPLE 1 Investigation of Neutralizing Activity

In this example, we investigated neutralizing activity of HIV-1 usingvarious peptides, shown in Tables 5 and 6.

We added separately 50 μL of the above peptide; 50 μL of 200TCID⁵⁰ ofHTLV-IIIB (laboratory strain) and KK-1(freshly isolated HIV, Ohtake etal. Kansenshou(Japanese), 64,1284-1294,1990) in each; and 50 μL of theabove peptide precisely diluted by 2 times step-by-step in 96-wellmicroplate, and mixed. Then, we used AZT as a positive control.

After incubation for 30 minutes at 37° C., 100 μL of MT-4 cellsuspension of 3×10⁴ cells was added and incubated for 6 days at 37° C.under 98% of humidity and 5% of CO₂. After the incubation, cellplasmodium effect (CPE) by HIV-1 prolification, namely when a medicinewas added with dilution step-by-step and infection cells gathered andbecame a state to form an island(focus form), was judged as an intensityof neutralizing activity(content to inhibit infection) on a pre-stage ofthis diluted magnification. These results are shown jointly in Tables 5and 6.

TABLE 5 HIV neutralizing Amino acid sequence activity (μg/ml) No. A1 A2A3 A4 A5 HThV-IIIB KK-1 1 Val Lys Lys Ala Gly Nglu 15.6 2 Asp Asp LysAla Gly 62.5 NE 3 Val Val Lys Ala Gly 31.3 NE 4 Aps Asp Val Ala Gly 500NE 5 Asp Asp Asp Ala Gly 1,000 1,000 6 Val Lys Val Ala Gly 125 NE 7 AspTyr Lys Ala Ala 31.3 125 Nos. 1-7 of Table 5 correspond to SEQ ID No. 1.

TABLE 6 Anti-HLV neutralizing Amino acid sequence activity (μg/ml) No.a1 a2 a3 a4 a5 HTLV-IIIB KK-1 1 Phe Tyr Arg Lys Ala 250 125 2 Tyr ArgArg Ala Ala 250 125 3 Trp Trp Glu Ala Ala 250 NE 4 Gly Tyr Tyr Lys Ala31.3 125 5 Tyr Tyr Ala Ala Ala 250 250 6 Phe Arg Lys Ala Ala 125 125 7Tyr Tyr Lys Lys Ala 312.5 312.5 8 Tyr Tyr Lys Leu Leu 31.3 62.5 9 TyrArg Lys Ala Ala 312.5 156.3 10 Tyr Tyr Lys Ala Ala 78.1 39.1 11 Arg TyrLys Ala Ala 62.5 31.25 12 Phe Tyr Ala Ala Ala NE 250-125 13 Tyr Ala LysAla Ala 500 NE 14 His Tyr Lys Ala Ala NE 500 15 Tyr Arg Met Ala Ala31.25 31.25 16 Tyr Tyr Met Ala Ala NE 125 17 Tyr Val Lys Ala Ala NE 25018 Arg Arg Trp Ala Tyr 39.1 39.1 19 Tyr His Lys Ala Ala 500 500 Nos.1-19 of Table 6 correspond to SEQ ID No. 4. Note) HTLV-IIIB; Laboratorystrain KK-1; Freshly isolated strain from domestic HIV patients NE; Noeffect

As is clear from the result of Tables 5 and 6, neither peptide which wasnot satisfied with a matter of this invention showed neutralizationactivity to HIV-1, while the peptides which satisfied it showed superioractivity. Then, a peptide of this invention shown in Table 5 presentsNo.1 peptide of this invention, and the peptide of Table 6 shows theNo.2, in each. In addition to the laboratory strain, when this inventedpeptide showed superior activity to the freshly isolated strain, thissuggested it would be extremely useful at a practical level beyond thelaboratory.

EXAMPLE 2 Agglutinin Test

In this example, we used the peptide shown in Table 7-10, and evaluatedit by an agglutinin test for the affinity to gp120.

After mixing equally both a suspension of 1% activation latex beads(Polyscience Inc., particle size is 0.2 mm) and avidin(10 mg/mL). Weincubated for 1 hour at 37° C. After the incubation, we added bovineserum albumin(BSA, 1 mg/mL) in it and carried out blocking of thereactive site for 30 minutes at 37° C. Then, after removing the bindingreagent by centrifuging repeatedly, biotinylated peptide (10 mg/mL inphosphate buffer, pH7.5) was added to each, and anti-gp120 agglutininreagent was prepared by incubation for 1 hour at 37° C.

As a positive control, we used colloidal gold conjugated by recombinantgp120 that was expressed in baculo virus(Immunodiagnostics Inc.,particle size is 30 nm), while as a negative control, we used thisrecombinant gp120 that was dissolved in phosphate buffer.

20 μ of the above agglutinin reagent and positive control were added andmixed on a test disk separately, we investigated it with the naked eyeafter standing for 10 minutes. The results are shown jointly in Tables 7to 10. When we used a negative control instead of a positive control inthis enforcement example, we confirmed that the agglutination was notobserved.

TABLE 7 Amino acid sequence Agglutinin test No. A1 A2 A3 A4 A5gp120/C.G. 1 Asp Val Lys Ala Gly +++ 2 Asp Lys Val Ala Gly + 3 Lys ValAsp Ala Gly +++ 4 Asp Asp Lys Ala Gly + 5 Lys Asp Asp Ala Gly ++ 6 ValAsp Asp Ala Gly ++ 7 Asp Val Asp Ala Gly + 8 Val Val Lys Ala Gly + 9 ValVal Asp Ala Gly 10 Lys Val Val Ala Gly + 11 Asp Tyr Lys Ala Ala + 12 AspPhe Lys Ala Gly ++ 13 Asp Trp Lys Ala Gly ++ 14 Asp Val Ar Ala Gly + 15Glu Val Lys Ala Gly ++ 16 Gly Gly Asp Val Lys Ala Gly 17 Gly Asp Val LysAla Gly ++ 18 Val Ile Asp Ala Gly + 19 Val Leu Asp Ala Gly + 20 Gly ValLys Ala Gly + 21 Asn Val Lys Ala Gly +++ 22 Asp Val Lys Trp Ala + 23 AspVal Lys Gly Lys + 24 Asp Val Lys Gly Trp + 25 Asp Val Lys Gly Leu + 26Asp Val Lys Gly Pro + Nos. 1-15 of Table 7 correspond to SEQ ID No. 1.No. 16 of Table 7 corresponds to SEQ ID No. 7. No. 17 of Table 7corresponds to SEQ ID No. 8.

TABLE 8 Amino acid sequence Agglutinin test No. A1 A2 A3 A4 A5gp120/C.G. 27 Asp Val Lys Ala Val + 28 Asp Val Lys Ala Ile + 29 Asp ValLys Ala Ser + 30 Asp Val Lys Ala Thr + 31 Asp Val Lys Ala Met + 32 AspVal Lys Ala Gln + 33 Asp Val Lys Ala Asn + 34 Asp Val Lys Ala His + 35Asp Val Lys Ala Arg + 36 Asp Val Lys Ala Phe + Nos. 27-36 of Table 8correspond to SEQ ID No. 1.

TABLE 9 Amino acid sequence Agglutinin test No. a1 a2 a3 a4 a5gp120/C.G. 1 Phe Tyr Arg Lys Ala + 2 Trp Trp Glu Ala Ala + 3 Tyr Gln GluAla Ala + 4 Gly Tyr Tyr Lys Ala + 5 Trp Trp Lys Ala Ala +++ 6 Tyr TyrLys Lys Ala + 7 Tyr Arg Lys Ala Ala + 8 Tyr Tyr Lys Ala Ala + 9 Arg TyrLys Ala Ala + 10 Tyr Ala Lys Ala Ala + 11 Tyr Tyr Gln Ala Ala + 12 TyrTrp Lys Ala Ala + 13 Gly Tyr Tyr Lys Ala Ala + 14 Trp Tyr Lys Ala Ala +15 Tyr Gln Lys Ala Ala ++ 16 His Tyr Lys Ala Ala + 17 Tyr Arg Tyr AlaAla ++ 18 Gly Tyr Ala Tyr Arg Lys + 19 Arg Arg Trp Ala Tyr + 20 Arg TyrTyr Lys Ala Ala + 21 Tyr Lys Lys Ala Ala + 22 Tyr His Lys Ala Ala + 23Asp Tyr Lys Ala Ala + 24 Tyr Tyr Lys Trp Ala + 25 Tyr Tyr Lys Gly Ala +26 Tyr Tyr Lys Ala Gly + Nos. 1-12, 14-17, 19 and 21-26 of Table 9correspond to SEQ ID No. 4. No. 13 of Table 9 corresponds to SEQ ID No.9. No. 18 of Table 9 corresponds to SEQ ID No. 10. No. 20 of Table 9corresponds to SEQ ID No. 11.

TABLE 10 Amino acid sequence Aggiutinin test No. a1 a2 A3 a4 a5gp120/C.G. 27 Tyr Tyr Lys Lys Ala + 28 Tyr Tyr Lys Val Ala + 29 Tyr TyrLys Ile Ala + 30 Tyr Tyr Lys Ser Ala + 31 Thr Tyr Lys Thr Ala + 32 TyrTyr Lys Met Ala + 33 Tyr Tyr Lys Gln Ala + 34 Tyr Tyr Lys Asn Ala + 35Tyr Tyr Lys His Ala + 36 Tyr Tyr Lys Phe Ala + 37 Tyr Tyr Lys Trp Ala +38 Tyr Tyr Lys Arg Ala + 39 Tyr Tyr Lys Ala Val + 40 Tyr Tyr Lys AlaIle + 41 Tyr Tyr Lys Ala Ser + 42 Tyr Tyr Lys Ala Thr + 43 Tyr Tyr LysAla Met + 44 Tyr Tyr Lys Ala Gln + 45 Tyr Tyr Lys Ala Asn + 46 Tyr TyrLys Ala His + 47 Tyr Tyr Lys Ala Phe + 48 Tyr Tyr Lys Ala Trp + 49 TyrTyr Lys Ala Arg + Nos. 27-49 of Table 10 correspond to SEQ ID No. 4.Note) gp120/C.G.; gp120 conjugated colloidal gold Degree ofagglutinination: +++ > +++ > +(agglutinination), −(no agglutinination)

From the results in Tables 7-10, we can confirm that each peptide shownin this invention has a superior affinity to gp120. The peptide of thisinvention is shown in Tables 7 and 8 is the No.1 peptide in thisinvention, and it shown in Tables 9 and 10 is the No. 2 peptide of it.

EXAMPLE 3 Effect of A4 and A5 in this Invention on the Agglutinin Test

To examine the effect of A4 and A5 amino acid on the aggluitinin test,the chain length of the No.1 peptide in TABLE 7 was changed. Theagglutinination was measured the same as in 2. These results are shownjointly in TABLE 11. Face note, ± means “agglutinination in tracedegree”.

TABLE 11 Amino acid sequence Agglutinin test No. A1 A2 A3 A4 A5gp120/C.G. 1 Asp Val Lys Ala Gly +++ 2 Asp Val Lys Ala — + 3 Asp Val Lys— — ± No. 1 of Table 11 corresponds to SEQ ID No. 1. No. 2 of Table 11corresponds to SEQ ID No. 12. No. 3 of Table 11 corresponds to SEQ IDNo. 13.

From Table 11, both No. 3 with only 3 amino acids (there are no A4 andA5 amino acids) and in No. 2 with only 4 amino acids(there are no A5amino acids) neutralizing activity decreased remarkably compared to No.1 with five amino acids. Namely, by the amino acid numerical decrease,the neutralizing activity has a tendency to fall, and particularly No.3, having only three amino acids.

EXAMPLE 4 Effect of a4 and a5 in this Invention on Neutralizing Activity

To examine the effect of a4 and a5 amino acids on neutralizing activity,the chain length of No. 8 in Table 9 were changed. The activity wasmeasured the same as in example 1. Their results are shown in Table 12.Face note, NE means “NO EFFECT”.

TABLE 12 Anti-HIV neutralizing Amino acid sequence activity (μg/ml) No.a1 a2 A3 a4 a5 HTLV-IIIB KK-1 1 Tyr Tyr Lys Ala Ala 78.1 39.1 2 Tyr TyrLys Ala — NE 250 3 Tyr Tyr Lys — — NE NE No. 1 of Table 12 correspondsto SEQ ID No. 4. No. 2 of Table 12 corresponds to SEQ ID No. 14. No. 3of Table 12 corresponds to SEQ ID No. 15.

From TABLE 12, both No. 3 with only 3 amino acids(there is not a4 and a5amino acid) and No. 2 with only 4 amino acids(there is not a5 aminoacids) neutralizing activity decreased remarkably compared to No.1 withfive amino acids. Namely, by amino acid numerical decrease, theneutraizing activity has a tendency to fall, and particularly, No.3having three amino acids.

EXAMPLE 5 Effect of a4 and a5 in this Invention on Neutralizing Activity

To examine the effect of a4 and a5 on neutralizing activity, No.1peptide in TABLE 13 was used as a positive control, while we used apeptide(No.2) that kind of a4 amino acids was changed to leucine,hydrophobic amino acid of same as alanine, or a peptide(No.3) that waschanged to proline. Then, we examined the effect to neutralizingactivity as in EXAMPLE 1. The results are shown jointly in TABLE 13.

TABLE 13 Anti-HIV neutralizing Amino acid sequence activity (μg/ml) No.a1 a2 a3 a4 a5 HTLV-IIIB KK-1 1 Tyr Tyr Lys Ala Ala 78.1 39.1 2 Tyr TyrLys Leu Leu 31.3 62.5 3 Trr Tyr Lys Pro Pro NE NE Nos. 1-2 of Table 13correspond to SEQ ID No. 4. No. 3 of Table 13 corresponds to SEQ ID No.16.

From TABLE 13, the kinds of a4 and a5 amino acids differed from theamino acid that was identified by this invention on No.1, theneutralizing activity decreased or disappeared. The results suggest thatthese kinds of a4 and a5 amino acid have an important effect on theexpression of the activity.

EXAMPLE 6 Effect of A4 and A5 in this Invention on Agglutinin

We examined the effect of A4 and A5 on agglutinin test as shown in TABLE14. The No.1 peptide was used as a positive control, while we used apeptide (No.2) that kind of A4 amino acids was changed to proline,hydrophobic amino acid of same as alanine, or a peptide (No.3) that waschanged to aspartic acid, acidic amino acid; similarly, the peptide(No.4) was changed to praline, and the peptide (No.5) was changed toglutamic acid. Then, we examined the effects on neutralizing activitythe same as in EXAMPLE 2. The results are shown jointly in Table 14.Face note, ±means “an agglutinin in trace degree”.

TABLE 14 Agglutinin test Amino acid sequence Colloidal No. A1 A2 A3 A4A5 gold conjugated gp120 1 Asp Val Lys Ala Gly +++ 2 Asp Val Lys Pro Gly− 3 Asp Val Lys Asp Gly − 4 Asp Val Lys Ala Asp − 5 Asp Val Lys Ala Glu± No. 1 and 4 of Table 14 correspond to SEQ ID No. 1. No. 2 of Table 14corresponds to SEQ ID No. 17. No. 3 of Table 14 corresponds to SEQ IDNo. 18. No. 5 of Table 14 corresponds to SEQ ID No. 19.

From TABLE 14, when a kind of A4 and A5 amino acid on No. 1 wereexchanged to a different amino acid, the agglutinin was negative, ordecreased to a trace degree. These results suggest that the kind of A4and A5 amino acids have important effects on agglutinin test.

EXAMPLE 7 Effect of Macromolecularization on Neutralizing Activity

We examined the effect of the peptide binding macromolecule onneutralizing activity. An inactive alkaline phosphatase (Alp) was usedas the macromolecule, and it was bound to the peptide that was preparedin SYNTHESIS 6, the same as in EXAMPLE 1. No.8 peptide was used as apositive control on TABLE 3, while unbound inactive alkaline phophatasewas used as negative control. These results are shown jointly in TABLE15. Face note, NE means “negative”.

TABLE 15 Anti-HIV neutralizing activity(μg/ml) HTLV-IIIB KK-1Macro-molecule Inactive Alp binding 125-250 62.5 peptide Positivecontrol peptide 125 125 Negative control inactive Alp NE NE

From TABLE 15, when the peptide in this invention bound to proteinalready known and grew up to a macromolecule, the solubility improvedand the neutralization activity increased too.

EXAMPLE 8 The Antibody-like Effect by Doing Induction of the PeptideMore than Once

After the peptide binding α-cyclodextrin prepared in SYNTHESIS 2 inhuman serum was suspended, we examined whether we could detect with acommercial diagnosis medicine kit for HIV(Dinabot Co., ┌HIV-1/HIV-2EIA┘). The judgment principle of this HIV diagnosis medicine kit wasthat it detects an anti-HIV antibody formed in patient serum, andparticularly, it's good point is that it can detect IgM antibodyappearing at an early stage of HIV infection. The result is shown inFIG. 1.

As is shown in FIG. 1, the above diagnosis medicine, which detects onlyanti-HIV antibodies present in HIV patient's serum, can detect dependingon the content peptide induced binding to α-cyclodextrin. From thisresult, the peptide in this invention has an affinity to HIV, and itclearly showed an antibody like effect when induction of the peptide wasdone more than once.

EXAMPLE 9 The Affinity of the Peptide to gp120(1)

The peptide bound to Sephadex 6 MB that was prepared in SYNTHESIS 7 wasadded to the various kinds of density prepared horseradish peroxidase(HRLP) labeled HIV-1-gp120(Immuno Diagnosis Co.) and enzyme unlabeledHIV-1-gp120 previously in each and constant disassociation(kd) ofpeptide in this invention calculated by drawing up A Schacherd Plot wecalculated was kd=2.14×10⁻¹⁰ M.

From this result, it is clear that the peptide in this invention has anaffinity of equal to, or greater than the antibody.

EXAMPLE 10 An Affinity of the Peptide to gp120(2)

The peptide binding Sephadex 4B that was prepared in SYNTHESIS 7 wasadded to the various kinds of density prepared horseradish peroxidase(HRP) and labeled HIV-1-gp120(Immuno Diagnosis Co.) and enzyme unlabeledHIV-1-gp120 previously in each and constant disassociation(kd) of thepeptide in this invention calculated by drawing up a Schacherd Plot wecalculated was kd=4.97×10⁻¹⁰M.

From this result, it is clear that the peptide in this invention has anaffinity of equal to, or greater than the antibody.

EXAMPLE 11 A Number of Recognition Site of the Peptide to gp120 (1)

To confirm the specificity of the peptide in this invention, we made“peptide conjugated latex beads” that labeled peptide of No.1 in TABLE 1and No.6 in TABLE 3 on red colored latex beads(Polyscience Co., 200 nmin diameter). When pseudo HIV-1 solution(gp120 conjugated colloidalgold) was added in 200 μl of this latex beads solution, redagglutination that could be observed by the naked eye promptly andeasily appeared. When the unlabelled gp120 solution(1 μg protein/ml)that was not conjugated with colloidal gold was added to it, it did notagglutinate at all. For this reason, we inferred that the peptide inthis invention bound only to the location of one site in gp120. Theresults are shown in TABLE 16.

TABLE 16 Colloidal gold conjugated gp120 gp120 protein Agglutinin testprotein contents(1 μg/ml) contents(1 μg/ml) No. 1 in Table 1 PresenceAbsence No. 6 in Table 3 Presence Absence

EXAMPLE 12 The Specificity Test

By using “peptide conjugated latex beads”, latex aggutination testmedicine of 2 kinds of peptide (No. 1 in Table and No. 6 in Table 3)that were prepared with Enforcement example 7, we examined whethernonspecific binding existed. The virus that was in use in thisenforcement example was serum, laboratory strain and a freshly isolatedstrain that was gathered from patients of hepatitis C or B at finalstage. Then, we used virus lysate that conjugated colloidal gold aspreviously. Titer or number (No.) used virus and presence ofagglutination are shown in TABLE 17.

TABLE 17 Presence of agglutinin Table 1 Table 3 No. Sample/Virus Titeror No. No. 1 No. 6 1 HIV-1 IIIB 1.0 × 10⁵ TCID₅₀ + + 2 HIV-1 Lav 1 1.0 ×10⁵ TCID₅₀ + + 3 HIV-1 kk-1 1.0 × 10^(4.5) TCID₅₀ + + 4 HIV-2 Lav2 1.0 ×10⁶ TCID₅₀ − − 5 Serum of HCV patients indistinct − − 6 Serum of HBVpatients indistinct − − 7 Hepatitis B surface 1.6 × 10⁵ units − −antigen(HbsAg) 8 Valaricella Zoster valirus 9,5 × 10⁵ units − − 9Argubella Zoster 4.9 × 10⁵ units − − valirus(VZV) 10 HTLV-1 virul lysate1.1 × 10⁵ units − − 11 human cytomegalovirus 5.5 × 10⁵ units − − (HCMV)12 Epstein-Barr virus 9.9 × 10⁵ units − − (EB virus)

From TABLE 17, it is clear that the affinity of peptide in thisinvention to gp120 was specific.

EXAMPLE 13 Removal of HIV from the Serum by an HIV Absorbing Column

We prepared the removing and absorbing ability of HIV-1 by using thepeptide/Sepharose 4B in this invention that was prepared in SYSNTHESIS9. The quantity of the peptide introduced in this invention that wasconjugated to the above carrier was 5 mg per 1 ml in bed volume.

First, after 100 μl of the above absorbed carrier was suspended inPBS(pH7.2) was added to a test tube, it was treated with an autoclavefor 30 minutes at 121° C., and decompressed naturally and the suppliedsample was prepared. In addition, as a virus model of pseudo HIV-1, weused gp120 conjugated with colloidal gold solution that was preparedusing a protein content of gp120 in 1.5 mg/mL The supplied virussolution, the pseudo HIV solution, was suspended with 100% human serum(96% as final content in serum), as a control the same content of pseudoHIV solution was suspended with PBS(pH 7.2, 0% human serum content). Thesupplied virus solution of 24(2.4 mL) volume was added to a test tubewith the antibody-like peptide of 1 volume(100 μl), and was mixed byshaking in a water bus for 2 hours at 37° C. After the incubation, wetook this test tube out and stood it for 30 minutes at room temperature,then, we collected the unabsorbed pseudovirus sample from thissupernatant fluid. We measured the rate of absorption the sample of 540nm, which was provided in this way; the rate of the 94% serum againstthe absorption of the 0% serum (was calculated as 100% in TABLE 18).These results show in TABLE 18.

TABLE 18 Content of serum(%) Rate of absorption(%)  0 100 94  74

EXAMPLE 14 Removal of HIV from the Serum through the use of an HIVAbsorbing Column

By using a carrier of cellulose series that covalented with the peptidein this invention prepared in SYNTHESIS 7, we examined the absorptionand removal of HIV. At this time, the rate of peptide in this inventionintroduced here was about 5 mg per 1 mL of the bed volume.

First, 100 μl of the above absorbed carrier suspended in PBS(pH7.2) wasadded to the test tube, it was treated with an autoclave for 30 minutesat 121° C., decompressed naturally, and was prepared as supplied sample.Another, HIV-1 virus solution was also used, kk-1 strain, this wasfreshly isolated from domestic AIDS patients by Ohtake et al.(Kansenshouzasshi(in Japanese), 64;1284-1294,1990) and frozen. After having rapidlydefrosted this KK-1 strain (1×10⁵ TCID₅₀), we ultra-centrifuged it andprepared the supplied sample that was suspended previously in 100% ofinactivated human serum. Next, 1 bed volume then, 24 volume of thesupplied virus solution were passed through the column. After theaddition, we washed down the serum 5 times against the bed volume toexpel the unbound virus that stayed in the column. The virus content wasmeasured by using p24 Antigen ELISA Kit (Cellular products Inc.), wecalculated S/CO from Cut Off value based on instructions attached to thekit. The samples measured were: the supplied sample (virus solution),the unbound sample, the washing solution, and extraction that lyzedbinding virus from the carrier (fraction of absorbed column) by lyzingbuffer, attached in the kit. The fraction that passed without binding tothe column included both this unbound sample and the washing solution.These results are shown in TABLE 19.

TABLE 19 p24(S/CO) Virus solution(Starting materials) 2,350 Fraction ofcolumn without 2,209 absorption(column passing solution withoutabsorption) Fraction of column absorption 205

From TABLE 19, we can understand easily that a little less than 10% ofthe supplied virus was absorbed by the column.

In the above EXAMPLE, we described the reason for the adoptedmeasurement method of p24 content to evaluate the quantity of the virus.

In this example, we used KK-1 strain, freshly isolated and suspended in100% human serum for the reason that it resembles the state of the virusin an AIDS patient. To evaluate the content correctly is very difficult.Even if it is usable in the gp120 ELISA Kit in the HIV laboratorystrain, it cannot be used with a freshly isolated virus such as KK-1.Rather, this is the reason that we used this virus. Accordingly, we canmeasure p24 content by the EIA method where there is a core proteinhaving a consensus sequence on first structure or measuring by theRT-PCR method. However, both methods have the weak point that theymeasure p24 including the debris. As mentioned above, HIV itself is veryunstable and will be broken with time, even during operation. It isthought the debris exists already, and it will increase. Therefore, whenthe debris increases compared with content of the absorbed virus, wecannot understand the result, even if we adopted both methods.Accordingly, a judgment by an infection experiment is desirable in orderto measure the content of the virus precisely. But, the freshly isolatedKK-1 that was used in this example is difficult to infect compared witha laboratory strain such as HIV-1 IIIB etc., and particularly, a virussolution of high density is needed, far exceeding the content of columnabsorption in the infection experiment. Considering these problems, itis very difficult to do the experiment. So, for the reason that it isdifficult to measure the absorption content if the content is in adilute virus solution of this degree, we measured the p24 content inthis example and calculated S/CO by comparing it with other data.

EXAMPLE 15 The Binding of HIV to Latex Conjugated Peptide

We filmed the agglutination condition of HIV by No.2 virus/No.1peptide-latex beads in TABLE 17. In detail, after the HIV-1LAV1laboratory strain was added to the above aggulutinin test medicine, itstood for 6 hours at 4 C. Afterwards, this agglutinated solution was puton a support membrane for observation by electron microscope, it wasnegatively stained with uranyl acetate and a photograph was taken.Referentially, we show this photograph in FIG. 2

From FIG. 2, we can observe that the HIV virus binds tightly to thelatex beads conjugated HIV-gp120 affinity peptide, those beads bindmutually and agglutinate.

As described above, the peptide in this invention is; superior instability, an affinity to gp120, is extremely useful as a medicine totreat HIV as it has neutralizing activity equal to previous antibodymolecules, can be used as to diagnose HIV through agglutination and is amedical tool for removing HIV. It has physical stability and isresistant to autoclave treatment, which is not so of antibody molecules.

19 1 5 PRT Artificial Sequence Description of Artificial SequenceSynthetic Peptide 1 Xaa Xaa Xaa Xaa Xaa 1 5 2 5 PRT Artificial SequenceDescription of Artificial Sequence Synthetic Peptide 2 Xaa Xaa Xaa XaaXaa 1 5 3 5 PRT Artificial Sequence Description of Artificial SequenceSynthetic Peptide 3 Xaa Xaa Xaa Xaa Xaa 1 5 4 5 PRT Artificial SequenceDescription of Artificial Sequence Synthetic Peptide 4 Xaa Xaa Xaa XaaXaa 1 5 5 5 PRT Artificial Sequence Description of Artificial SequenceSynthetic Peptide 5 Xaa Xaa Xaa Xaa Xaa 1 5 6 5 PRT Artificial SequenceDescription of Artificial Sequence Synthetic Peptide 6 Xaa Xaa Xaa XaaXaa 1 5 7 7 PRT Artificial Sequence Description of Artificial SequenceSynthetic Peptide 7 Gly Gly Asp Val Lys Ala Gly 1 5 8 6 PRT ArtificialSequence Description of Artificial Sequence Synthetic Peptide 8 Gly AspVal Lys Ala Gly 1 5 9 6 PRT Artificial Sequence Description ofArtificial Sequence Synthetic Peptide 9 Gly Tyr Tyr Lys Ala Ala 1 5 10 6PRT Artificial Sequence Description of Artificial Sequence SyntheticPeptide 10 Gly Tyr Ala Tyr Arg Lys 1 5 11 6 PRT Artificial SequenceDescription of Artificial Sequence Synthetic Peptide 11 Arg Tyr Tyr LysAla Ala 1 5 12 4 PRT Artificial Sequence Description of ArtificialSequence Synthetic Peptide 12 Asp Val Lys Ala 1 13 3 PRT ArtificialSequence Description of Artificial Sequence Synthetic Peptide 13 Asp ValLys 1 14 4 PRT Artificial Sequence Description of Artificial SequenceSynthetic Peptide 14 Tyr Tyr Lys Ala 1 15 3 PRT Artificial SequenceDescription of Artificial Sequence Synthetic Peptide 15 Tyr Tyr Lys 1 165 PRT Artificial Sequence Description of Artificial Sequence SyntheticPeptide 16 Tyr Tyr Lys Pro Pro 1 5 17 5 PRT Artificial SequenceDescription of Artificial Sequence Synthetic Peptide 17 Asp Val Lys ProGly 1 5 18 5 PRT Artificial Sequence Description of Artificial SequenceSynthetic Peptide 18 Asp Val Lys Asp Gly 1 5 19 5 PRT ArtificialSequence Description of Artificial Sequence Synthetic Peptide 19 Asp ValLys Ala Glu 1 5

What is claimed is:
 1. An isolated peptide having affinity to gp120represented by formula (1): H-Asp-Val-Lys-Ala-Gly-R (SEQ ID No. 1)wherein, in the formula, H is hydrogen; and R is OH derived from acarboxyl group or NH₂ derived from an acid amide group.
 2. An isolatedpeptide having affinity to gp120 represented by formula (2):A1′-Val-Lys-Ala-Gly-R (SEQ ID No. 2) wherein, in the formula, A1′ isaspartic acid, lysine, valine, glutamic acid, glycine, asparagine, or atyrosine residue; and R is OH derived from a carboxyl group or NH₂derived from an acid amide group.
 3. An isolated peptide having affinityto gp120 represented by formula (3): H-Asp-Val-Lys-Ala-A5′-R (SEQ ID No.3) wherein, in the formula, H is hydrogen; A5′ is glycine, alanine,valine, leucine, isoleucine, serine, threonine, methionine, asparagine,glutamine, histidine, lysine, arginine, phenylalanine, tryptophan,proline, or a tyrosine residue; and R is OH derived from a carboxylgroup or NH₂ derived from an acid amide group.
 4. An isolated peptidehaving affinity to gp120 consisting of up to 10 amino acids andcomprising the amino acid sequence of Asp-Val-Lys-Ala-Gly (SEQ ID No.1).
 5. The peptide according to claim 4 having affinity to gp120represented by formula (4): H-Gly-Asp-Val-Lys-Ala-Gly-R (SEQ ID No. 4),wherein in the formula, H is hydrogen; and R is OH derived from acarboxyl group or NH₂ derived from an acid amide group.
 6. The peptideaccording to claim 4 having affinity to gp120 represented by formula(5): H-Gly-Asp-Val-Lys-Ala-Gly-R (SEQ ID No. 5), wherein in the formula,H is hydrogen; and R is OH derived from a carboxyl group or NH₂ derivedfrom an acid amide group.
 7. A compound, comprising the peptide asclaimed in any one of claims 1 to 6, bound to a macromolecule compoundor a medicinal compound.
 8. The compound as claimed in claim 7, whereinsaid macromolecule compound comprises a synthetic polymer or abiopolymer.
 9. The compound as claimed in claim 7, wherein the medicinalcompound is AZT.
 10. A compound, comprising the peptide as claimed inany one of claims 1 to 6, or a pharmaceutically acceptable salt thereof.11. The compound as claimed in claim 10, wherein the peptide is bound toa carrier.
 12. A viral agglutination test compound, comprising thepeptide as claimed in any one of claims 1 to
 6. 13. A viral test kit,comprising the viral agglutination test compound of claim
 12. 14. Amethod of removing HIV viruses comprising the steps of: contacting thecompound as claimed in claim 11, with a liquid sample solutioncontaining the HIV viruses; and absorbing and removing the HIV viruseswhich have affinity for the compound.